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Volume :1
Issue :
23
of October
02
2013 |
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| | COMPARISION OF TWO DNA EXTRACTION METHODS
USING THE RT-PCR | | | Author Name:FAHRI GAVAZAJ1, ILIA MIKEREZI2, BAHRIJE GAVAZAJ2,
VALON MORINA2, BEKIM SAMADRAXHA3, FATMIR CAKAJ3 |  | | Show Author Profile | | Abstract: The rapid development of molecular genetic analysis tools has made it possible
to analyze most biological material even they are in a small quantity. Many different
extraction protocols are being used to isolate DNA. However not all extraction methods
perform equally well with heavily inhibited factors and sometimes is very important to
choose an extraction procedure that could extract DNA efficiency and had the ability to
minimize the amount of inhibitors co-extracted with the sample.
Two different extraction methods were chosen: Chelex and Organic Extraction.
To evaluate the efficiency of the procedures, quantity of DNA is compared using TaqMan
Probe & Absolute Quantification method in ABI Prism RT-PCR. The samples used were
blood samples.
The results showed that with both methods extraction was efficiency for further
analysis, but concentration of DNA was greater extracted with Chelex than it with
Organic Extraction.
Compare with Organic Extraction, Chelex is more efficient, more rapid,
inexpensive, involves fewer steps and thus fewer opportunities for sample to sample
contamination, no hazard chemicals are used. |
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| | YOGIC PRACTICES CAN IMPROVE THE BLADDER
FUNCTIONS IN INCOMPLETE SPINAL CORD
INJURY PATIENTS | | | Author Name:ANVITA SINGH, V.P. SHARMA , J.K. TRIVEDI, RAKESH SHUKLA,
SUNITA TIWARI AND KAMAKHYA KUMAR | | | Show Author Profile | | Abstract: Objective: To evaluate the effect of Yogic practices on Bladder functioning in
incomplete Spinal cord injured patients.
Methods: This was a randomised control study. The study was conducted in the
Department of Physical Medicine and Rehabilitation, C.S.M.M.U., Lucknow. A total of
60 patients were interviewed using a pre-tested semi-structured schedule between 16 to
60 years of age, diagnosed study cases of traumatic incomplete spinal cord injury (SCI)
with neurological (both sensory & motor) impairment below thoracic (T12 level) with
bladder & bowel involvement were included in the study. The duration of injury was
more than 2 months, were divided randomly using random number table into 2 groups
study group (received Yoga) (n=30) and control group (not received Yoga) (n=30) for 6
months. Patients were asked to come on 3rd and 6th month. Anthropometric
measurements were also recorded. The paired t-test was used to compare the changes
amongst follow-ups and unpaired t-test was used to compare between groups. The pvalue<
0.05 was considered as significant.
Results: At the baseline both the groups were similar in anthropometric and
clinical parameters The bladder function score was insignificantly lower (p>0.05) in
study group (1.43±0.57) as compared to controls at the baseline (1.73±0.83) and it is
increased to 3.13 (±0.78) in study group after 6 months of Yoga and became to 2.13
(±0.78) in control group. The change in bladder function score was significantly
(p<0.0001) higher in study group as compared to s control group from baseline to 3 and
6 months. There was 54.3% increase in bladder function score from baseline to 6 months
in study group which was 18.8% in control group.
Conclusion: The finding of the study reveals that there is significant impact of
Yoga in the management of Bladder among spinal cord injured patients. |
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